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The CKChip system
Extending the reach of single cell research
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The CKChip system is a technology that enables fluorescence-based imaging of thousands of individual living cells, each held at a given position on the Chip. The system allows monitoring, sequential manipulation and simple analysis of both adherent and non-adherent cells held on the chip. The CKChip system opens new opportunities for high content analysis of non-adherent cells, and enables acquiring kinetic data of drug action, response to stimulators, Ca++ flux or gene expression simultaneously from multiple single cells over time
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Tracking of non-adherent living cells made easy
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The CKChip system effortlessly deposits single living cells of any kind into wells arranged as a matrix. This solves the problem of locating and revisiting non-adherent living cells, opening the way for prolonged kinetic observation and quantitative imaging on demand.
With this platform, selected cells of interest, or groups of thousands can be continuously and simultaneously tracked, measured, manipulated and analyzed, with their individual identities verified by unique locations on the grid
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Unparalleled cell order allows unique experimental freedom
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The CKChip's well design ensures that many cells remain viable while anchored in their registered locations. This allows various levels of freedom in cell manipulation:
- Anchoring non-adherent living cells
- Applying different reagents in sequence
- Documenting cell dynamics for extended time periods
- Detecting and revisiting specific cells of interest
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Software dedicated exclusively to CKChip analysis
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The exploratory capacity of the CKChip is further enhanced with WELLS: a proprietary, user-friendly dedicated software package that simplifies quantitative analysis procedures.
WELLS enables convenient analysis of the data acquired by means of the CKChip
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Three components work together to enable simple cell monitoring and analysis:
- CKChip : a cell carrier platform that holds cells securely in place during repeated stainings, washings and treatments with reagents. Individual cell addresses are registered, and microscope navigation is enabled with "landmark wells" that serve as grid coordinates.
- CK Loader: a device that safely distributes living cells across the CKChip micro-wells and draws each cell into its own well.
- CK WELLS : a user-friendly dedicated image analysis software for easy analysis and cross-referencing of kinetic data acquired with the CKChip.
To the starter kit...
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Advantages of cell based assays performed with the CKChip system
o Non-adherent cells can be easily analyzed for dynamic cell response
On the CKChip, cells are held in defined, coded positions, thus facilitating imaging and analysis of non-adherent cells.
o Deep and versatile analytical capacity
The CKChip enables gathering, continuous monitoring, and analyzing cell dynamics at the single cell level, providing valuable information not easily attainable by flow cytometry or fluorometry:
- Detection of responding cells even when they comprise a small minority of the population
- Increased probability of detecting rare cells or rare cell events
- Identification of cell sub-populations defined by the dynamics of cell response
- Revealing transient and subtle changes of cell response
- Reliable statistics from analysis of thousands of individual cells
o Multiplexing cell-based kinetic assays
- Reagents can be exchanged in the course of imaging to expose cells sequentially to different stimulants
- Cells analyzed for [Ca++]i dynamics by the DynamiCa kit can be further analyzed for other parameters (apoptosis, cell surface markers, intracellular protein expression, and the like)
o User friendly
- Easy to learn, even for microscopy novices
- Easy to use, requiring only common lab skills
- Easy to analyze, included software is intuitive and automatic
To facilitate microscopic analysis of multiple single cells over time:
1. Prepare the CKChip starter kit.
2. Prepare relevant fluorescent probes compatible with microscopic analysis.
3. Read the Quick Start Guide carefully.
4. Load cells on the CKChip (included in the CKChip starter kit).
5. Stimulate cells on the CKChip.
6. Image cells over the required period of time.
7. Analyze images automatically with the CKChip software (included in the CKChip starter kit).
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