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DynamiCa – The CKChip Calcium Assay Kit
Your way to reveal previously undetected information
The CKChip DynamiCa kit offers a simple yet powerful platform facilitating microscopic analysis of transient intracellular calcium levels.
By combining the CKChip technology fluorescence microscopy and image analyses, simultaneous monitoring of thousands of individual cells over time is enabled. On the CKChip, numerous single cells are held in defined physical positions. Cells are then loaded with a calcium-sensitive fluorescence probe, stimulated, and imaged over time. Cell images are then automatically analyzed with the included CKChip image-analysis software to unravel changes of [Ca++]i in multiple individual cells simultaneously over time.
Researchers use our products to discover heterogeneous calcium dynamics in sub-populations of cells, to unravel rare cells, and to detect transient changes of [Ca++]i in both adherent and non-adherent cells.
Advantages of calcium assays performed with the DynamiCa kit
o Non-adherent cells can be easily analyzed for dynamic changes of [Ca++]i
On the CKChip, cells are held in defined, coded positions, thus facilitating imaging and analysis of non-adherent cells.
o Deep and versatile analytical capacity
The CKChip enables gathering, continuous monitoring, and analyzing [Ca++]i dynamics at the single cell level, providing valuable information not easily attainable by flow cytometry or fluorometry:
- Detection of responding cells even when they comprise a small minority of the population
- Increased probability of detecting rare cells or rare cell events
- Identification of cell sub-populations defined by calcium dynamics
- Revealing transient and subtle changes of [Ca++]i dynamics
- Detection of calcium oscillations
- Reliable statistics from analysis of thousands of individual cells
o Multiplexing cell-based kinetic assays
- Reagents can be exchanged in the course of imaging to expose cells sequentially to different stimulants
- Cells analyzed for [Ca++]i dynamics by the DynamiCa kit can be further analyzed for other parameters (apoptosis, cell surface markers, intracellular protein expression, and the like)
o User friendly
- Easy to learn, even for microscopy novices
- Easy to use, requiring only common lab skills
- Easy to analyze, included software is intuitive and automatic
To facilitate microscopic analysis of intracellular calcium levels:
1. Prepare the DynamiCa calcium dynamics assay kit.
2. Prepare the calcium fluorescent probe compatible with microscopic analysis.
3. Read the DynamiCa Quick Start Guide carefully.
4. Load cells on the CKChip (included in the DynamiCa kit).
5. Stimulate cells on the CKChip.
6. Image cells over the required period of time.
7. Analyze images automatically with the CKChip software (included in the DynamiCa kit).
| Catalog number |
Product name |
CKChip type |
Grid type |
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| CKPA0800024 |
DynamiCa Calcium assay kit |
CKChip-R |
A20 |
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| CKPA0800050 |
DynamiCa Calcium assay kit |
CKChip-R |
A50 |
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| CKPA1000024 |
DynamiCa Calcium assay kit |
CKChip-I |
A20 |
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| CKPA1000050 |
DynamiCa Calcium assay kit |
CKChip-I |
A50 |
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